The fine came as part of the DFS' investigation into electronic trading of foreign exchange and FX electronic trading systems in the period 2009 to 2014.
In August, it was reported that nine banks would pay a total of $2bn (£1.3bn) to US investors after being accused of conspiring to manipulate foreign exchange rates.
In a statement, Barclays said that the civil penalty will be reflected in its Q4 2015 results and that it is continuing to co-operate with other ongoing investigations.